IL-17和HSP90在不同牛肝中的定位和定量研究

doi:10.11843/j.issn.0366-6964.2019.09.018

摘要/Abstract

摘要： 本试验旨在比较研究不同牛肝组织中IL-17和HSP90的分布及表达差异。分别选取5例健康成年牦牛、犏牛及黄牛，取肝组织，采用HE染色、免疫组织化学染色及实时荧光定量研究肝组织学结构特点、蛋白阳性定位及基因定量表达。HE染色结果显示，肝细胞呈索状排列，肝索之间的肝血窦中有少量的淋巴细胞和枯否细胞；免疫组织化学染色结果显示，IL-17和HSP90在牦牛、犏牛及黄牛肝细胞的细胞质中表达；免疫组化半定量和实时荧光定量的结果一致，在犏牛肝表达量最高，牦牛次之，黄牛最低（P <0.01）。IL-17和HSP90基因在牦牛、犏牛和黄牛肝中表达的差异说明具有种属特异性；IL-17和HSP90蛋白半定量阳性结果说明相对于黄牛肝，犏牛和牦牛肝对温差变化等刺激具有更好的适应性。

Abstract: The aim of this study was to study the distribution and quantification of IL-17 and HSP90 in liver tissues of different cattle, and to compare the positive reaction and differential expression of IL-17 and HSP90 in the liver tissues of Yak, Cattle-yak and Yellow cattle. Liver samples of healthy adult Yak, Cattle-yak and Yellow cattle (n=5) were selected, respectively. HE, immunohistochemical, and Quantitative Real-time PCR methods were used to observe the histological features, protein positive localization, and gene quantification of liver, respectively. Results showed that:hepatic cells were arranged in a cord-like manner, and a small number of Lymphocytes and Kupffer's cells were found in the blood sinuses between the hepatic cords (HE staining). Both IL-17 and HSP90 were expressed in the cytoplasm of liver cells of Yak, Cattle-yak and Yellow cattle (Immunohistochemical results). The results of Immunohistochemistry Semi-quantitative, and Quantitative Real-time PCR were consistent. The highest expression was found in the liver of Cattle-yak, followed by Yak, and the lowest in Yellow cattle (P < 0.01). Differential expression of IL-17 and HSP90 genes in liver of Yak, Cattle-yak and Yellow cattle shows species specificity. Semi-quantitative positive results of IL-17 and HSP90 protein showed that the liver of Yak and Cattle-yak had better adaptability to temperature differences than the liver of Yellow cattle.

中图分类号:

S852.1

赵凯, 崔燕, 刘鹏刚, 何俊峰, 宋亮丽, 廖博, 杨晓晴. IL-17和HSP90在不同牛肝中的定位和定量研究[J]. 畜牧兽医学报, 2019, 50(9): 1897-1903.

ZHAO Kai, CUI Yan, LIU Penggang, HE Junfeng, SONG Liangli, LIAO Bo, YANG Xiaoqing. Localization and Expression of IL-17 and HSP90 in Different Bovine Livers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1897-1903.

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参考文献

[1] WIENR G, JIANLIN H, RUIJUN L. The yak[M]//ULLREY D E, BAER C K, POND W G. Encyclopedia of Animal Science. Boca Raton, FL:CRC Press, 2010.
[2] HIROTA K, DUARTE J H, VELDHOEN M, et al. Fate mapping of IL-17-producing T cells in inflammatory responses[J]. Nat Immunol, 2011, 12(3):255-263.
[3] WANG L, LI Q, WANG L, et al. The role of Th17/IL-17 in the pathogenesis of primary nephrotic syndrome in children[J]. Kidney Blood Press Res, 2013, 37(4-5):332-345.
[4] TANG Y, BIAN Z, ZHAO L, et al. Interleukin-17 exacerbates hepatic steatosis and inflammation in non-alcoholic fatty liver disease[J]. Clin Exp Immunol, 2011, 166(2):281-290.
[5] 叶艳萍, 宋雷英. 谷氨酰胺肠内营养+低分子肝素对重症肺炎患者全身炎症反应程度的影响[J]. 海南医学院学报, 2018, 24(15):1406-1409.
YE Y P, SONG L Y. Effect of glutamine enteral nutrition + low molecular weight heparin on systemic inflammatory response in patients with severe pneumonia[J]. Journal of Hainan Medical University, 2018, 24(15):1406-1409. (in Chinese)
[6] ABUSLEME L, MOUTSOPOULOS N M. IL-17:overview and role in oral immunity and microbiome[J]. Oral Dis, 2017, 23(7):854-865.
[7] LINDQUIST S, CRAIG E A. The heat-shock proteins[J]. Ann Rev Genet, 1988, 22:631-677.
[8] SHI Q, DONG Z J, WEI H M. The involvement of heat shock proteins in murine liver regeneration[J]. Cell Mol Immunol, 2007, 4(1):53-57.
[9] WEI T, GAO Y H, WANG R X, et al. A heat shock protein 90β isoform involved in immune response to bacteria challenge and heat shock from Miichthys miiuy[J]. Fish Shellfish Immunol, 2013, 35(2):429-437.
[10] AMBADE A, CATALANO D, LIM A, et al. Inhibition of heat shock protein 90 alleviates steatosis and macrophage activation in murine alcoholic liver injury[J]. J Hepatol, 2014, 61(4):903-911.
[11] LIU P G, YU S J, CUI Y, et al. Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue[J]. PLoS One, 2017, 12(7):e0179321.
[12] YANG X, CUI Y, YUE J, et al. The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens)[J]. PLoS One, 2017, 12(5):e0176451.
[13] LIU P G, YU S J, CUI Y, et al. Regulation by HSP27/P53 in testis development and sperm apoptosis of male cattle (cattle-yak and yak)[J]. J Cell Physiol, 2019, 234(1):650-660.
[14] WU L, WANG C H, BOISSON B, et al. The differential regulation of human ACT1 isoforms by HSP90 in IL-17 signaling[J]. J Immunol, 2014, 193(4):1590-1599.
[15] LIU J J, ZHAO H J, WANG Y, et al. Impacts of simultaneous exposure to arsenic (Ⅲ) and copper (Ⅱ) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus[J]. Int Immunopharmacol, 2018, 64:60-68.
[16] HAMMERICH L, HEYMANN F, TACKE F. Role of IL-17 and Th17 cells in liver diseases[J]. Clin Dev Immunol, 2011, 2011:345803.
[17] WANG L Y, CHEN S J, XU K S. IL-17 expression is correlated with hepatitis B-related liver diseases and fibrosis[J]. Int J Mol Med, 2011, 27(3):385-392.
[18] NAKAGAWA S I, UMEHARA T, MATSUDA C, et al. HSP90 inhibitors suppress HCV replication in replicon cells and humanized liver mice[J]. Biochem Biophys Res Commun, 2007, 353(4):882-888.
[19] HE W, HU H X. BⅡB021, an HSP90 inhibitor:a promising therapeutic strategy for blood malignancies[J]. Oncol Rep, 2018, 40(1):3-15.
[20] MOVITA D, VAN DE GARDE M D B, BIESTA P, et al. Inflammatory monocytes recruited to the liver within 24 hours after virus-induced inflammation resemble Kupffer cells but are functionally distinct[J]. J Virol, 2015, 89(9):4809-4817.
[21] ARMENDARIZ-BORUNDA J, SEYER J M, POSTLETHWAITE A E, et al. Kupffer cells from carbon tetrachloride-injured rat livers produce chemotactic factors for fibroblasts and monocytes:the role of tumor necrosis factor-α[J]. Hepatology, 1991, 14(5):895-900.
[22] MONTEIRO M, ALMEIDA C F, AGUA-DOCE A, et al. Induced IL-17-producing invariant NKT cells require activation in presence of TGF-β and IL-1β[J]. J Immunol, 2013, 190(12):5909-5910.
[23] BARIN J G, BALDEVIANO G C, TALOR M V, et al. Macrophages participate in IL-17-mediated inflammation[J]. Eur J Immunol, 2012, 42(3):726-736.
[24] CYPOWYJ S, PICARD C, MARÍDI L, et al. Immunity to infection in IL-17-deficient mice and humans[J]. Eur J Immunol, 2012, 42(9):2246-2254.
[25] CHEN D H, LUO X P, XIE H Y, et al. Characteristics of IL-17 induction by Schistosoma japonicum infection in C57BL/6 mouse liver[J]. Immunology, 2013, 139(4):523-532.
[26] DU W J, ZHEN J H, ZENG Z Q, et al. Expression of Interleukin-17 associated with disease progression and liver fibrosis with hepatitis B virus infection:IL-17 in HBV infection[J]. Diagn Pathol, 2013, 8:40.
[27] VAZQUEZ-TELLO A, HALWANI R, HAMID Q, et al. Glucocorticoid receptor-beta up-regulation and steroid resistance induction by IL-17 and IL-23 cytokine stimulation in peripheral mononuclear cells[J]. J Clin Immunol, 2013, 33(2):466-478.
[28] RITOSSA F. Discovery of the heat shock response[J]. Cell Stress Chaperones, 1996, 1(2):97-98.
[29] CHENG C H, YANG F F, LING R Z, et al. Effects of ammonia exposure on apoptosis, oxidative stress and immune response in pufferfish (Takifugu obscurus)[J]. Aquat Toxicol, 2015, 164:61-71.
[30] TUKAJ S, WEGRZYN G. Anti-HSP90 therapy in autoimmune and inflammatory diseases:a review of preclinical studies[J]. Cell Stress Chaperones, 2016, 21(2):213-218.
[31] PEZZULO A A, TUDAS R A, STEWART C G, et al. HSP90 inhibitor geldanamycin reverts IL-13 and IL-17-induced airway goblet cell metaplasia[J]. J Clin Invest, 2019, 129(2):744-758.
[32] KUMAR P, KOLLS J K. Act1-hsp90 heats up T_H17 inflammation[J]. Nat Immunol, 2013, 14(1):16-17.